Figure 1.

In vitro reconstitution of a memory switch comprising CaMKII and N2Bs. (A) In vitro reconstitution system with a Ctl peptide (GluN2B 1095−1119), where CaMKII functions as a memoryless switch (left), and with N2Bs (GluN2B 1289−1310), where CaMKII functions as a memory switch (right). (B) Ca²⁺ pulse stimulation (black); 20 μM Ca²⁺ for 10 min and 0.56 μM (basal Ca²⁺ concentration) afterward (top). T286 phosphorylation of CaMKII (T286P) with Ctl (bottom, left) or with N2Bs (bottom, right) in response to the pulse stimulation (solid circles) and to the basal Ca²⁺ concentration (open circles). The CaMKII kinase inhibitor (K252a) (dark blue circles) or control buffer (light blue circles) was added at 3 h. Error bars indicate mean ± SD (n ≥ 3). To see this figure in color, go online.