Table 2.

EF-G concentration dependence in unlabeled subpopulation analysis of GS1-GS2 equilibrium

EF-G	0 nM	5 nM	50 nM	500 nM	1000 nM
${\rho }_{+\text{EF}-\text{G}}$	0.13	0.30	0.56	0.65	0.67
$\text{Δ}{G}_{+\text{EF}-\text{G}}$	1.7	1.2	1.3	1.4	1.4
$\text{Δ}{G}_{-\text{EF}-\text{G}}$	−2.4	−1.7	−0.8	−0.4	−0.4

Fraction of EF-G bound complexes, ${\rho }_{+\text{EF}-\text{G}}$, and the free energy difference between the GS1 and GS2 state, $\text{Δ}G$, for the bound and unbound subpopulation, as a function of EF-G concentration.