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. 2014 Mar 15;53(13):2126–2135. doi: 10.1021/bi401597m

Figure 6.

Figure 6

(A) Sedimentation equilibrium experiments with SwHaCE in solution. Using rotor speeds of 9000, 14000, and 18000 rpm (which are equal to 6500g, 15800g, and 26100g, respectively), and following protein at 280 nm, the calculated molecular mass of SwHaCE is 144.3 kDa, indicating a dimer in solution at equilibrium. (B) Sedimentation equilibrium experiment for SwHaCE in solution with the SwH-NOX Fe(II)–NO complex. Using rotor speeds of 9000, 14000, and 18000 rpm (which are equal to 6500g, 15800g, and 26100g, respectively), and following protein complexes at 400 nm, the calculated molecular mass for the complex is 197.2 kDa at equilibrium. This matches the molecular mass expected for a heterotetramer of two SwH-NOX proteins and two SwHaCE proteins. Each experiment was conducted in triplicate.