Table 1. Summary of Sedimentation Equilibrium Results and Controls.
| protein/protein complex | calculated molecular mass (theoretical molecular mass) (kDa)f |
|---|---|
| HaCE by itselfa,b | 144.3 (149.0, dimer) |
| HaCE with WT H-NOX Fe(II)–NOa,c | 197.2 (193.0, 2+2 heterotetramer) |
| HaCE with WT H-NOX Fe(II)a,d | 198.0 (193.0, 2+2 heterotetramer) |
| HaCE with E16K H-NOX Fe(II)–NOa,c | 76.0 (−) |
| WT H-NOX Fe(II)–NOa,c | 75.0 (−) |
| WT H-NOX Fe(II)–NOe,c | 23.3 (22.5, monomer) |
| WT H-NOX Fe(II)e,d | 23.0 (22.5, monomer) |
| E16K H-NOX Fe(II)–NOe,c | 23.8 (22.5, monomer) |
a
The sedimentation equilibrium run was conducted at three rotor speeds calculated for the molecular mass of SwHaCE (74.5 kDa): 9000 rpm (6520g), 14000 rpm (15800g), and 18000 rpm (26100g).
b
The scans for the sedimentation equilibrium runs were collected at 280 nm.
c
The scans for the sedimentation equilibrium runs were collected at 400 nm.
d
The scans for the sedimentation equilibrium runs were collected at 430 nm.
e
The sedimentation equilibrium run was conducted at three rotor speeds calculated for the molecular mass of SwH-NOX (22.5 kDa): 17000 rpm (23300g), 27000 rpm (58700g), and 34000 rpm (93100g).
f
HeteroAnalysis and Sednterp were used to calculate the molecular mass.