Figure 5.

Effect of tNAD⁺ and UQ₀ on NADH-UQ₀ oxidoreductase activity with NADH the varied substrate. The reaction mixture contained 5.0 nM NDH-2, 0.2 mM NADH, and 2.0 mM UQ₀ in the absence (○) or presence (●) of 1.0 mM tNAD⁺. Data were fit globally using eq 4 assuming competitive inhibition.