Video 1. The video shows an ABACUS1-2µ Arabidopsis root, growing in a RootChip16, exposed to pulses of increasing concentrations of ABA.
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DOI: 10.7554/eLife.01741.016
The RATIO and YFP videos show the root throughout the experiment whereas the central charts show the average ratio (in magenta) and intensity (in yellow) of three regions (ROIs shown as overlaid on the root). The RATIO video (left) shows the ratio (DxAm/DxDm) using the color-code shown in the calibration bar on the leftmost side. The YFP video (right) shows acceptor emission intensities upon acceptor excitation (AxAm). Grey areas in the charts indicate ABA pulses of increasing concentrations (0.1–312.5 µM (+)-ABA, in 5x increments and supplied as racemic mix). The red vertical line shows position of current time in the charts. Scale bar corresponds to 100 µm and the counter in the lower left corner shows time elapsed in minutes.