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S. typhimurium WaaJ preferentially uses UDP-Glc but not UDP-Gal. Gal-Glc-Hep2-1-deP-KLA (labeled C) and UDP-Gal were reacted with S. typhimurium WaaI (0.1 mg/mL) to produce Gal-[Gal]-Glc-Hep2-1-deP-KLA (labeled Ds). After 90 min, S. thyphimurium WaaJ was added and incubated for the times indicated in the presence of UDP-Gal or UDP-Glc. The product labeled Es is proposed to be Glc-Gal-[Gal]-Glc-Hep2-1-deP-KLA.
