Figure 7.

Time dependence of the E. coli K-12 WaaO reaction. Hep₂-1-deP-KLA (labeled A) was reacted with WaaG (0.01 mg/mL) and WaaB (0.1 mg/mL) for 285 min as described in the legend of Figure 6. E. coli K-12 WaaO (0.1 mg/mL) and 1 mM MgCl₂ were added, and a portion of the reaction mixture was spotted onto a TLC plate at the times indicated. WaaO catalyzes the formation of a single glucose addition (labeled D) and at increased times additional glucoses (labeled D*).