Table 1. Equilibrium Binding Constants for DB1255 and Its Analogues in the Presence of ATGA and AATT^a.

	KD (nM)
compound	ATGA	AATT
DB293b	114; 32	45
DB1255	26	86
DB1282	416	1219
DB1998	24	33

^aThe experiments were conducted in cacodylic acid buffer (pH 6.25) at 25 °C.

^bK₁ and K₂ are the first and second equilibrium binding constants, respectively, obtained from a 2:1 cooperative binding model for DB293 with ATGA. Other compounds bind to both DNAs as 1:1 complexes. Dissociation constants were calculated from SPR experiments.