Table 1. Equilibrium Binding Constants for DB1255 and Its Analogues in the Presence of ATGA and AATTa.
KD (nM) |
||
---|---|---|
compound | ATGA | AATT |
DB293b | 114; 32 | 45 |
DB1255 | 26 | 86 |
DB1282 | 416 | 1219 |
DB1998 | 24 | 33 |
The experiments were conducted in cacodylic acid buffer (pH 6.25) at 25 °C.
K1 and K2 are the first and second equilibrium binding constants, respectively, obtained from a 2:1 cooperative binding model for DB293 with ATGA. Other compounds bind to both DNAs as 1:1 complexes. Dissociation constants were calculated from SPR experiments.