Table 4.
Evaluation of biological activity of burchellin against A. aegypti (L3)
|
A |
Mortality (L3 larvae) |
|
|---|---|---|
| X ± SD | % | |
| Control |
a |
0 |
| Control 2 |
b |
0 |
| 12 h |
1.66 ± 1.15 ab** |
17 |
| 24 h |
1.0 ± 0 |
10 |
| 48 h |
1.0 ± 0 |
3 |
| 72 h |
0 |
0 |
| 96 h |
0 |
0 |
| 120 h |
0 |
0 |
|
B |
Mortality (L3 larvae) |
|
| |
Number |
% |
| Control |
0 |
0 |
| Control 2 |
0 |
0 |
| 1 h |
0 |
0 |
| 2 h |
0 |
0 |
| 3 h |
0 |
0 |
| 24 h |
0 |
0 |
| 48 h | 60 | 100 |
After 12-120 h (4A) and 1-48 h (4B) incubation, larvae in mid-development were treated with burchellin. Treatment of A. aegypti with 15 μg/mL burchellin using 10 L3 larvae per group (4A) and 30 μg/mL burchellin using 100 larvae (L3) per group (4B). Larvae (n = 10) were removed at intervals of 1 - 48 h (4B). All experiments were performed in triplicate. Values are mean ± standard deviation (X ± SD). Values followed by the same letter did not differ significantly; Tukey test, P > 0.05. Significance levels are represented as **P < 0.01 vs. Control 2 = Acetone control.