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Characterization of cultured human epidermal stem cells (ESCs). (A) Holoclone formation of rapidly adherent cells cultured up to 1 week (inverted phase contrast microscope×200). (B) and (C) Representative double-labeled immunostaining of the holoclone, using the antibodies directed against the mouse β1-integrin and K19 (original magnification×400). Red indicates positive staining for β1-integrin. Blue indicates positive staining for K19.
