Figure 2.

Schematic of co-capture assay. The minimal HIV-1 provirus with an intron interrupted reverse Gaussia luciferase gene as a reporter and the desired glycoproteins were transfected into 293FT cells to produce virus. The virus was then captured by antibodies against the glycoproteins or no antibody in the wells of an ELISA plate. Unbound virus was washed away from all wells except a straight infectivity positive control. Cells susceptible to infection mediated by one or the other of the glycoproteins were then added to the wells and two days later luciferase production was assayed.