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Effects of EEPC on LPS-induced IL-1β and TNF-α expression in RAW 264.7 macrophages. The cells were pre-treated with various concentrations of EEPC for 1 h before LPS treatment (0.5 μg/ml), and total RNA was isolated at 6 h after LPS treatment. The levels of IL-1β and TNF-α mRNA were determined using RT-PCR. GAPDH was used as an internal control.
