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. 2014 Mar 15;14:193. doi: 10.1186/1471-2407-14-193

Table 1.

VM signaling-related markers

Gene PCR primers (forward-reverse) Amplification size (bp) Cycle no.
PI3-K
5′-TGTCGCAGCCCAGGTAGATT-3
269
35
5′-CAGGAGGTGGTCGGGTCAAG3-′
MMP-2
5′-TCTGAGGGTTGGTGGGATTGG-3
290
35
5′-AAGAGCGTGAAGTTTGGAAGCA-3′
MM1-MMP
5′-CAAAGGCAGAACAGCCAGAGG3-′
180
35
5′-ACAGGGACCAACAGGAGCAAG-3′
Ln-5γ2
5′-ACACGGGAGATTGCTACTCG3-′
123
35
5′-ACCCATTGTGACAGGGACAT-3′
GAPDH 5′-CCTCTATGCCAACACAGTGC-3′
211 35 ~ 40
5′GTACTCCTGCTTGCTGATCC-3′