Table 2.
Recombinase | Mutations | Core sequence |
|||
---|---|---|---|---|---|
20B | 20S | 20G | 20T | ||
β | None | −− | −− | −− | |
M94V | +++ | −− | −− | ||
N95D | ++++ | −− | −− | ||
M94T, R104H | + | −− | −− | ||
M94V, N107S | ++++ | −− | − | ||
V58A, N95D | + | −− | −− | ||
M94T, N95D | +++ | −− | −− | ||
E71G, M94V, N95D | ++ | −− | −− | ||
N68S, E71G, V88A, N95D | +++ | −− | −− | ||
K33R, N49S, E71G, N95D | +++ | −− | −− | ||
R18P, R41P, D55G, R67G, E71G, M94I, N107K, Y114N | +++ | −− | −− | ||
Sin | None | −− | −− | −− | |
I2V | ++ | −− | −− | ||
Q115R | + | −− | −− | ||
Q87R, Q115R | ++++ | −− | −− | ||
T32A, N97D, Q115R | + | −− | − | ||
I11V, D12N, V78A, Q115R | ++++ | −− | − | ||
I11V, D12N, V78A, Q115R, L150P | + | −− | −− | ||
T77I, D85G, K110R, I133V, V138I | + | −− | −− | ||
I11T, V61A, D85G, K110R, I113T, Q115R | ++ | −− | −− | ||
I64A, V78A, K84R, I90V, I113T, Q115R, Q137R | ++++ | −− | − | ||
V53A, E76G, E83G, D85G, V99A, N102S, I113S, Q115R | ++++ | −− | − |
Symbols indicate recombination efficiency. ++++, >35% recombination; +++, 20–35%; ++, 6–19%; +, 1–5%; −, <0.1%; −−, <0.01%. The limit of detection of recombination by split gene reassembly is ∼10−5%. All Sin variants are derived from the I100T background strain.