Table 2.
Recombination by selected β and Sin catalytic domains
| Recombinase | Mutations | Core sequence |
|||
|---|---|---|---|---|---|
| 20B | 20S | 20G | 20T | ||
| β | None | −− | −− | −− | |
| M94V | +++ | −− | −− | ||
| N95D | ++++ | −− | −− | ||
| M94T, R104H | + | −− | −− | ||
| M94V, N107S | ++++ | −− | − | ||
| V58A, N95D | + | −− | −− | ||
| M94T, N95D | +++ | −− | −− | ||
| E71G, M94V, N95D | ++ | −− | −− | ||
| N68S, E71G, V88A, N95D | +++ | −− | −− | ||
| K33R, N49S, E71G, N95D | +++ | −− | −− | ||
| R18P, R41P, D55G, R67G, E71G, M94I, N107K, Y114N | +++ | −− | −− | ||
| Sin | None | −− | −− | −− | |
| I2V | ++ | −− | −− | ||
| Q115R | + | −− | −− | ||
| Q87R, Q115R | ++++ | −− | −− | ||
| T32A, N97D, Q115R | + | −− | − | ||
| I11V, D12N, V78A, Q115R | ++++ | −− | − | ||
| I11V, D12N, V78A, Q115R, L150P | + | −− | −− | ||
| T77I, D85G, K110R, I133V, V138I | + | −− | −− | ||
| I11T, V61A, D85G, K110R, I113T, Q115R | ++ | −− | −− | ||
| I64A, V78A, K84R, I90V, I113T, Q115R, Q137R | ++++ | −− | − | ||
| V53A, E76G, E83G, D85G, V99A, N102S, I113S, Q115R | ++++ | −− | − | ||
Symbols indicate recombination efficiency. ++++, >35% recombination; +++, 20–35%; ++, 6–19%; +, 1–5%; −, <0.1%; −−, <0.01%. The limit of detection of recombination by split gene reassembly is ∼10−5%. All Sin variants are derived from the I100T background strain.