Table 3.
Target site | Gene | Core sequence | Recombination (%) |
---|---|---|---|
β wild-type | CAAT AGA GT AT AC TTA TTTC | 44 ± 9 | |
β consensus | TRGA NRG GT AT AC CYN TCYA | ND | |
β degenerate | NNNV NNR GT WW AC YNN BNNN | ND | |
β-AT 1 | CNGB3 | AAAC AGA GT AT AC CCTCATT | 92 ± 63 |
β-AT 2 | CNGB3 | GTTACTA GT AT AC TTA TCCT | 37 ± 34 |
β-AT 3 | Factor VIII | GTGCCAG GT AT AC TGTGTTA | 36 ± 9 |
β-AT 4 | CNGB3 | TAACTCA GT AT AC TTGGGGG | 32 ± 10 |
β-AT 5 | Factor VIII | TGGGGGA GT AT AC CTT TTTC | 27 ± 4 |
β-AT 6 | BCR | ACTCTTG GT AT AC TGTTCTG | 3.2 ± 1 |
β-TT 1 | RPE | CAGGTGA GT TT AC CAA TCTG | 0.07 ± 0.008 |
β-TT 2 | CNGA3 | AAAAGAG GT TT AC TCA GCTC | 0.03 ± 0.02 |
β-TT 3 | CNGB3 | CTAATTA GT TT AC CAGTGAA | 0.03 ± 0.01 |
β-TT 4 | CNGB3 | GTCACCA GT TT AC CAT TTCT | 0.02 ± 0.01 |
β-TT 5 | CNGB3 | TTAA ATA GT TT AC TGGTGCA | 0.01 ± 0.003 |
β-AA 1 | CNGB3 | GAGA GTG GT AA AC TACCTGC | 0.01 ± 0.004 |
β-AA 2 | Factor IX | GAGACAG GT AA AC TAGGCAG | <0.01 |
β-TA 1 | Factor VIII | TGAAGTG GT TA AC TAT GCAA | <0.01 |
β-TA 2 | CNGB3 | TTTCTAA GT TA AC TTT TTAC | <0.01 |
β-TA 3 | Factor VIII | CCTCTAG GT TA AC CAA TTTG | <0.01 |
β-TA 4 | CNGB3 | GGCCCTG GT TA AC TCTCCTC | <0.01 |
Dinucleotide core composition is denoted in target site (e.g. β-AT 1 contains an AT dinucleotide core). Positions 10-7, 6-4, 3-2, and the dinucleotide core are separated by spaces. Base mismatches between genomic and wild-type core sequences are underlined. Recombination was measured by split gene reassembly. ND indicates not determined. Error values indicate standard deviation (n = 3). Abbreviations for nucleotide substitutions are as follows: N = A, T, C, or G; V = A, C, or G; B = T, C, or G; R = A or G; Y = T or C; W = A or T.