Table 3.

β-Mediated recombination of core sequences derived from the human genome

Target site	Gene	Core sequence	Recombination (%)
β wild-type		CAAT AGA GT AT AC TTA TTTC	44 ± 9
β consensus		TRGA NRG GT AT AC CYN TCYA	ND
β degenerate		NNNV NNR GT WW AC YNN BNNN	ND
β-AT 1	CNGB3	AAAC AGA GT AT AC CCTCATT	92 ± 63
β-AT 2	CNGB3	GTTACTA GT AT AC TTA TCCT	37 ± 34
β-AT 3	Factor VIII	GTGCCAG GT AT AC TGTGTTA	36 ± 9
β-AT 4	CNGB3	TAACTCA GT AT AC TTGGGGG	32 ± 10
β-AT 5	Factor VIII	TGGGGGA GT AT AC CTT TTTC	27 ± 4
β-AT 6	BCR	ACTCTTG GT AT AC TGTTCTG	3.2 ± 1
β-TT 1	RPE	CAGGTGA GT TT AC CAA TCTG	0.07 ± 0.008
β-TT 2	CNGA3	AAAAGAG GT TT AC TCA GCTC	0.03 ± 0.02
β-TT 3	CNGB3	CTAATTA GT TT AC CAGTGAA	0.03 ± 0.01
β-TT 4	CNGB3	GTCACCA GT TT AC CAT TTCT	0.02 ± 0.01
β-TT 5	CNGB3	TTAA ATA GT TT AC TGGTGCA	0.01 ± 0.003
β-AA 1	CNGB3	GAGA GTG GT AA AC TACCTGC	0.01 ± 0.004
β-AA 2	Factor IX	GAGACAG GT AA AC TAGGCAG	<0.01
β-TA 1	Factor VIII	TGAAGTG GT TA AC TAT GCAA	<0.01
β-TA 2	CNGB3	TTTCTAA GT TA AC TTT TTAC	<0.01
β-TA 3	Factor VIII	CCTCTAG GT TA AC CAA TTTG	<0.01
β-TA 4	CNGB3	GGCCCTG GT TA AC TCTCCTC	<0.01

Dinucleotide core composition is denoted in target site (e.g. β-AT 1 contains an AT dinucleotide core). Positions 10-7, 6-4, 3-2, and the dinucleotide core are separated by spaces. Base mismatches between genomic and wild-type core sequences are underlined. Recombination was measured by split gene reassembly. ND indicates not determined. Error values indicate standard deviation (n = 3). Abbreviations for nucleotide substitutions are as follows: N = A, T, C, or G; V = A, C, or G; B = T, C, or G; R = A or G; Y = T or C; W = A or T.