Figure 6.

Level of RISC association of a miRNA is not predicted by the level of 3′ tailing. miRNAs bearing 3′ tails were identified by deep sequencing (Supplementary Tables S3 and S4), and the percentage of tailed miRNAs in the total small RNA and RISC-associated small RNA libraries was determined in 293 cells. These percentages are presented as a ratio of percentage tailed in Total vs. percentage tailed in the RISC-associated pool, with the miRNAs listed in descending order of RISC association. A similar, more extensive, analysis looking at all miRNAs expressed in 293 cells or A549 cells is presented in Supplementary Figure S3.