. 2014 Jan 21;42(7):4577–4589. doi: 10.1093/nar/gkt1403

Table 1.

The sequences of primers used to generate templates for in vitro transcription

Transcript	Primer	Primer sequence (5′–3′)
argX-hisR-leuT-proM precursor	FWD	ATCCTAATACGACTCACTATAGGGAACGGCGCTAAGCGCCCG
argX-hisR-leuT-proM precursor	RVS	AAAAAACCCCGCCGAAGCGG
5′ hisR to 3′	FWD	ATCCTAATACGACTCACTATAGGGGGTGGCTATAGCTCAGTTGG
5′ hisR to 3′	RVS	AAAAAACCCCGCCGAAGCGG
5′ hisR to 3′ proM	FWD	ATCCTAATACGACTCACTATAGGGGGTGGCTATAGCTCAGTTGG
5′ hisR to 3′ proM	RVS	TGGTCGGCGAGAGAGGAT
5′ leuT to 3′	FWD	ATCCTAATACGACTCACTATAGGGGCGAAGGTGGCGGAATTGGT
5′ leuT to 3′	RVS	AAAAAACCCCGCCGAAGCGG
5′–3′ leuT	FWD	ATCCTAATACGACTCACTATAGGGAACGGCGCTAAGCGCCCG
5′–3′ leuT	RVS	TGGTGCGAGGGGGGG
5′–3′ hisR	FWD	ATCCTAATACGACTCACTATAGGGAACGGCGCTAAGCGCCCG
5′–3′ hisR	RVS	TGGGGTGGCTAATGGGATT
5′ argX to 3′ hisR	FWD	ATCCTAATACGACTCACTATAGGGGCGCCCGTAGCTCAGCTG
5′ argX to 3′ hisR	RVS	TGGGGTGGCTAATGGGATT
5′–3′ proM	FWD	ATCCTAATACGACTCACTATAGGGAACGGCGCTAAGCGCCCG
5′–3′ proM	RVS	TGGTCGGCGAGAGAGGAT
metT-leuW-glnUW-metU-glnVX precursor	FWD	ATCCTAATACGACTCACTATAGGGCGCAACGCCGATAAGGTA
metT-leuW-glnUW-metU-glnVX precursor	RVS	ATTGAATGAACGCAGAAAAGC
glyVXY precursor	FWD	ATCCTAATACGACTCACTATAGGGCCGTAACGACGCAGAAATG
glyVXY precursor	RVS	GCGTCGCTGTGGATATTTTATT

The T7 polymerase promoter encoded in each of the forward primers is underlined.