Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Methyltransferase-directed alkylation of DNA is followed by CuAAC at the alkylated site. (1) DNA is incubated along with a DNA methyltransferase enzyme (targeting adenine N6 for modification here) and a synthetically prepared cofactor (AdoEnYn). The methyltransferase is able to catalyse the transfer of the extended alkyl chain (grey) to the DNA duplex. (2) These modified sites on the DNA are subsequently fluorescently labelled via the CuAAC click reaction.
