Table 1.

Quantitative analysis of the molecules used in fluorocode mapping

Methyltransferase	Ligand	Total number localized fluorophores	Average size of DNA molecule (kb)	Average number of fluorophores per molecule	Percent matched data	Percent matched reference sites
M.TaqI	TBTA	820	30	60	67	98.7
	THPTA	982	28	80	53	98.3
M.FokI	TBTA	471	25	44	52	93.0

The term ‘data’ refers to the experimentally derived fluorocode maps, whereas the ‘reference’ is the in silico-generated fluorocode map based on the known DNA sequence for the T7 bacteriophage genome. A ‘match’ between the ‘data’ and ‘reference’ is designated when a point (fluorophore) in the ‘data’ lies within 50 bp, i.e. in the same bin or an adjacent bin to a point on the reference map.