Figure 5.

Tagged MKT1 and PBP1 partially co-migrate with polysomes on sucrose gradients. Extracts from ∼5 × 10⁸ bloodstream-form trypanosomes were separated on sucrose gradients. Proteins were extracted and analysed by western blotting using antibodies to myc, RPS9 (small ribosomal subunit protein S9) and trypanothione reductase (TRYR). TRYR is a soluble cytosolic protein but seems to run throughout the gradients. (A and B): Cells expressing MKT1-myc; (C and D): Cells expressing PBP1-myc. (A and C): Cycloheximide (100 µg/ml) was added immediately prior to parasite lysis; (B and D): RNase A (1 g/ml) added immediately after lysis.