Table 3.
Activities of ZC3H11 and MKT1 fragments in the tethering assay
| Box B | Tet | CAT activity | CAT RNA | |
|---|---|---|---|---|
| ZC3H11 101–195 | + | – | 0.9 ± 0.1 | 0.8 ± 0.2 |
| + | + | 1.0 ± 0.1 | 0.8 ± 0.2 | |
| ZC3H11 101–202 | + | – | 1.0 ± 0.1 | 1.1 ± 0.3 |
| + | + | 1.0 ± 0.1 | 1.2 ± 0.2 | |
| ZC3H11 200–364 | + | – | 0.9 ± 0.1 | 1.6 ± 0.3 |
| + | + | 1.0 ± 0.0 | 1.1 ± 0.6 | |
| ZC3H11 186–364 | + | – | 1.0 ± 0.1 | 0.7 ± 0.2 |
| + | + | a3.5 ± 0.6 | a5.0 ± 0.6 | |
| MKT1 233–735 | + | – | 1.0 ± 0.1 | 1.3 ± 0.1 |
| + | + | 1.1 ± 0.1 | 1.9 ± 0.2 | |
| – | – | 1.0 ± 0.1 | 1.0 ± 0.1 | |
| – | + | 1.0 ± 0.1 | 1.1 ± 0.1 | |
| MKT1 1–494 | + | – | 0.6 ± 0.1 | 1.0 ± 0.2 |
| + | + | 0.5 ± 0.1 | 1.0 ± 0.2 | |
| – | – | 0.9 ± 0.1 | 1.1 ± 0.1 | |
| – | + | 0.9 ± 0.1 | 1.2 ± 0.1 | |
| MKT1 SRV571->AAA | + | – | 1.0 ± 0.2 | 1.0 ± 0.3 |
| + | + | a3.9 ± 0.6 | a3.8 ± 0.8 | |
| – | – | 1.0 ± 0.1 | 1.0 ± 0.2 | |
| – | + | 1.0 ± 0.0 | 1.1 ± 0.3 | |
| MKT1 D590A | + | – | 1.0 ± 0.0 | 0.9 ± 0.1 |
| + | + | a4.0 ± 0.6 | a4.4 ± 0.5 | |
| – | – | 1.0 ± 0.0 | 1.2 ± 0.2 | |
| – | + | 1.0 ± 0.1 | 1.2 ± 0.2 |
Results are arithmetic mean and standard deviation for at least three independent experiments. aIncreased CAT protein and RNA.