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. 2014 Jan 27;42(7):e53. doi: 10.1093/nar/gku082

Figure 2.

Figure 2.

Transgene expression from LVs and long-term GFP expression from aniLV-transduced 293T cells. (A) Time-course evaluation of GFP expression from the LV-transduced 293T cells at post-transduction time points. The population of GFP-positive cells was analyzed at intervals to measure the effect of wPRE replacement by S/MAR and functional integrase replacement by defective integrase. (B) Long-term stability of GFP expression from the aniLV-transduced and enriched cells after sorting. Mitotic stability and rate of gene expression from integration defective S/MAR containing LV in relation to control vectors. (C) Microscopy images of GFP florescence from the LV-transduced 293T cells. GFP signal was consistently lower in integrase-defective niLV vectors and S/MAR harboring vectors. Scale bars: 400 µm. (D) Comparison of GFP expression levels (MFI) from LV-transduced 293T cells two days post-transduction. GFP expression was significantly lower in integrase-defective wPRE-harboring vector than the integrating wPRE vector.