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. 2014 Jan 31;42(7):4697–4711. doi: 10.1093/nar/gku097

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© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Yhc1 R21A and K28A bypass the requirement for the essential PRP28 gene. Yeast prp28Δ yhc1Δ cells harboring the indicated YHC1 allele on a CEN HIS3 plasmid and either wild-type PRP28 (CEN LEU2) or an empty CEN LEU2 plasmid (prp28Δ) were grown in liquid cultures at 37°C to mid-log phase. The cultures were adjusted to A₆₀₀ of 0.1, and aliquots of serial dilutions were spotted to YPD agar. Photographs of the plates after incubation for 2 d (30, 34, 37°C) or 4 d (20, 25°C) are shown.