Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 31;42(7):4682–4696. doi: 10.1093/nar/gku098

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — Hfq and RydC both prevent ribosome loading onto the csgD mRNA. (A) Ribosome toeprint assays performed on csgD mRNA₂₁₅ in the presence of increasing amounts of RydC or purified Hfq. Left panel: 25- to 200-fold excess RydC as compared with csgD mRNA₂₁₅. Right panel: 0.4- to 4-fold excess purified Hfq as compared with csgD mRNA₂₁₅. The experimentally proven toeprints are indicated with asterisks, with their sizes reflecting the intensity of the toeprints. Plus/minus indicates the presence of purified ribosomes with the csgD mRNA; U, A, G and C: indications of the csgD mRNA₂₁₅ sequencing ladders. The SD sequence and AUG initiation codon of the csgD mRNA₂₁₅ are also indicated. (B) Schematic representation of the csgD mRNA 5′-domain, emphasizing the location of the ribosome toeprints (marked with asterisks) induced by either Hfq or RydC.