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. 2014 Jan 22;53(8):1263–1274. doi: 10.1021/bi4016583

Figure 1.

Figure 1

Changes in ThT fluorescence at 483 nm over time in the absence (black) and presence of Cu(II) (green), Zn(II) (blue), and Ni(II) (red). Each solution contained 100 μM β2m, 150 μM CuSO4 or ZnSO4 or 3 mM NiSO4, 500 mM urea, 150 mM potassium acetate, 25 mM MOPS (pH 7.4), and 80 μM ThT.