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SEC analyses of 100 μM β2m incubated with (a) 4 mM Ni(II), (b) 150 μM Zn(II), and (c) 150 μM Cu(II). In each case, the protein and indicated metal were mixed with 500 mM urea, 150 mM potassium acetate, and 25 mM MOPS (pH 7.4) at 37 °C. The control sample is 100 μM β2m incubated with 5 mM EDTA in 150 mM potassium acetate and 25 mM MOPS (pH 7.4) at 37 °C. M, M2, M4, and M6 indicate the β2m monomer, dimer, tetramer, and hexamer, respectively.
