Table 2. Percentages of Modified Residues Observed after a 20 min MCO Reaction of the β2m–Ni(II) Complex.
| MCOa | controlb | |
|---|---|---|
| N-terminus | 27.9 ± 3.6% | 0 ± 0% |
| His31 | 14.5 ± 1.9% | 0.1 ± 0.2% |
| His51 | 0.4 ± 0.2% | 0.2 ± 0.2% |
| Trp60 | 4.4 ± 2.0% | 8.4 ± 3.0% |
| Trp95 | 1.6 ± 1.2% | 1.7 ± 1.5% |
a
β2m (200 μM) was reacted with 250 μM NiSO4, 5 mM ascorbate, 0.5 mM persulfate in 150 mM potassium acetate, and 25 mM MOPS (pH 7.4).
b
In the control experiment, 200 μM β2m was reacted with 250 μM NiSO4 and 0.5 mM persulfate in 150 mM potassium acetate and 25 mM MOPS (pH 7.4).