. 2014 Jan 22;53(8):1263–1274. doi: 10.1021/bi4016583

Table 3. Hydrogen–Deuterium Exchange Analyses of 100 μM β2m Incubated in the Presence or Absence of 100 μM Zn(II) in D₂O Containing 150 mM Potassium Acetate and 25 mM MOPS (pH 7.4) at 37 °C.

		no. of deuteriums incorporated
residue	Zn(II)	1 day	2 days	4 days
His13	–	0.171 ± 0.004	0.32 ± 0.02	0.568 ± 0.005
	+	0.161 ± 0.009	0.31 ± 0.03	0.53 ± 0.03
His31	–	0.104 ± 0.008	0.25 ± 0.01	0.46 ± 0.02^a
	+	0.10 ± 0.01	0.241 ± 0.001	0.41 ± 0.03^a
His51	–	0.195 ± 0.007^a	0.33 ± 0.01^a	0.52 ± 0.02^a
	+	0.14 ± 0.02^a	0.24 ± 0.01^a	0.44 ± 0.03^a
His84	–	<0.05^b	<0.05^b	0.051 ± 0.001
	+	<0.05^b	<0.05^b	0.054 ± 0.006

Deuterium levels in the Zn-bound protein that are statistically different from the deuterium levels in the Zn-free protein based on a t test (p < 0.05).

Number of deuteriums incorporated into His84 at 1 and 2 days were too low to be confidently measured.

Table 3. Hydrogen–Deuterium Exchange Analyses of 100 μM β2m Incubated in the Presence or Absence of 100 μM Zn(II) in D2O Containing 150 mM Potassium Acetate and 25 mM MOPS (pH 7.4) at 37 °C.

Table 3. Hydrogen–Deuterium Exchange Analyses of 100 μM β2m Incubated in the Presence or Absence of 100 μM Zn(II) in D₂O Containing 150 mM Potassium Acetate and 25 mM MOPS (pH 7.4) at 37 °C.