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Population of the χ2 = 180° rotamer for Leu residues, determined from the 13Cmethyl chemical shifts. The hE:FOL:NADP+ and hE:THF:NADP+ complexes have essentially identical chemical shifts and therefore similar rotamer populations. The Leu side chains are assumed to populate only the χ2 = 180° or 60° rotamer (eq 4). Asterisks denote residues with a unique average χ2 rotamer conformation in one or more hDHFR complexes.
