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. 2014 Feb 4;136(8):3264–3270. doi: 10.1021/ja412934t

Figure 5.

Figure 5

(A) Cell lysates expressing SNAP-CA, SNAP-Bcl-xL, and SNAP-FKBP were individually labeled with one of three DNA sequences and combined with a cell lysate expressing SNAP and labeled with 256 DNA sequences. The pooled lysates were combined with a library of 262 DNA-linked small molecules, including DNA-linked GLCBS, CBS, Bad, Bak, BakL78A, and rapamycin, for a model library×library IDUP “selection”. (B) IDUP using a library of cell lysates expressing SNAP-target fusions identified all five known target+ligand pairs, including A - FKBP+rapamycin, B - Bcl-xL+Bad, C - Bcl-xL+Bak, D - CAII+GLCBS, and E - CAII+CBS, despite having affinities from 0.2 nM to 3.2 μM. (C) For interactions with Kd = 40 nM–26 μM, we observed a strong relationship between the log of target–ligand Kd and the number of sequence counts after selection.