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Lactose transport. Transport of [14C]lactose (10 mCi/mmol) of E. coli T184 expressing WT LacY, mutant N272D, N272E, N272K, N272Q, N272S, N272A, N272V, N272G, N272L, N272Y, N272F, N272W, V264A, or G268A, or no permease was measured at 0.4 mM lactose for given times as described in Materials and Methods. Expression of WT LacY and each mutant as determined by Western blotting.
