Table 1. KD Values for NPG Binding of LacY Mutants.
KDapp (μM)a | standard error | |
---|---|---|
WT | 19.2 | 1.1 |
N272A | 142.6 | 39.4 |
N272D | –b | –b |
N272E | –b | –b |
N272F | –b | –b |
N272Q | 20.0 | 1.8 |
N272S | –b | –b |
N272V | –b | –b |
V264A | 30.1 | 4.1 |
G268A | 154.7 | 57.3 |
Values determined as described in the legend of Figure 4.
Binding is too poor to determine a KD.