Figure 4.

Fluorescence of chemically synthesized peptides effected by modified and unmodified hASL^Lys3_UUU. An initial fluorescent signal (FS₀) of peptide alone (1.5 μM) was obtained. Then, a 2-fold excess of ASL was added to each peptide, and the fluorescent signal (FS₁) was monitored. The percent change (100·(FS₁/FS₀)) is graphed for each of the assayed peptides. Dark gray bars represent the percent change in fluorescence in the presence of the modified hASL^Lys3_UUU, and light gray bars represent the percent change in the presence of the unmodified hASL^Lys3_UUU. Sequences for P1–P38 are presented in Table 3