Table 4. Affinity of Peptides for hASLLys3UUUa.
| peptide | modified or unmodified hASLLys3UUU | Kd (μM) |
|---|---|---|
| P6 | modified | 0.50 ± 0.10b |
| unmodified | ID | |
| P27 | modified | ∼0.05 ± 0.02c |
| unmodified | ID | |
| P31 | modified | 0.58 ± 0.24 |
| unmodified | ID | |
| P35* | modified | 1.87 ± 1.00 |
| unmodified | ID |
a
ID = indeterminable; could not be curve fitted and calculated from the data because of little change in fluorescence with binding of ASL.
b
Kd value from previous studies.9
c
Kd value is approximate for it is one-tenth the concentration of the fluorescent peptide reporter.