Table 4. Prediction Accuracy of Destabilizing/Stabilizing Mutations and Binding Hot Spots for Single Mutations.

		destabilizing	stabilizing	binding hot spot
method	training/test set	accuracy	accuracy	accuracy	sensitivity	specificity
Pred2	SKEMPI/SKEMPI	0.95	0.11	0.80	0.51	0.91
	SKEMPI/NM	1.00	0.07	0.81	0.64	0.87
CC/PBSA	NM/NM	0.99	0.32	0.84	0.48	0.97
FoldX	test: NM	0.72	0.48	0.77	0.37	0.91
	test: SKEMPI	0.67	0.41	0.76	0.30	0.94
BeAtMuSiC	test: NM	0.95	0.23	0.77	0.33	0.92
	test: SKEMPI	0.90	0.18	0.77	0.30	0.95