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. 2014 Mar 24;136(13):4801–4804. doi: 10.1021/ja500979k

Figure 2.

Figure 2

CcTET oxidation assays at 37 °C under pH 5.8, 7.0, and 8.0. The reaction mix contains 1 μM 5mC containing dsDNA, 2 μM CcTET, 50 mM Bis-Tris, pH 5.8 (or HEPES, pH 7.0 or 8.0), 75 μM Fe(NH4)2(SO4)2, 2 mM ascorbic acid, 1 mM α-KG, and 2.5 mM DTT for 30 min at 37 °C and then quenched by heating to 100 °C for 5 min and immediately cooled in an ice bath.