Table 3. Manually Verified Protein Abundance Changes in Ntapi:gAHA1 vs Wild-Type Plasma Membrane Fractions.
| experiment
1 |
experiment
2 |
experiment
3 |
|||||
|---|---|---|---|---|---|---|---|
| accession | annotation | 14NTAPa/15NWT | 15NWT/14NTAPa | 14NTAPa/15NWT | 15NWT/14NTAPa | 14NTAPb/15NWT | 15NWT/14NTAPb |
| AT2G18960 | AHA1 | 1.06 (6)a | 0.79 (9) | 1.93 (6) | 0.62 (7) | 1.34 (9) | 0.70 (5) |
| p = 0.01b | p = 1 × 10–5 | p = 1 × 10–4 | |||||
| AT2G38940 | ATPT2 | 1.69 (4) | 0.88 (3) | 0.76 (2) | 1.08 (4) | 0.69 | |
| p = 0.05 | |||||||
| AT3G01290 | band 7 | 1.53 (6) | 0.69 (8) | 1.17 (4) | 1.05 (6) | 1.74 (7) | 0.47 (4) |
| p = 6 × 10–9 | p = 2 × 10–6 | ||||||
| AT1G66970 | SVL2 | 0.84 (7) | 1.37 (10) | 0.66 (8) | 1.57 (8) | 0.80 (8) | 0.95 (8) |
| p = 2 × 10–5 | p = 4 × 10–7 | ||||||
| AT1G72150 | PATL1 | 0.94 (7) | 1.55 (7) | 0.72 (6) | 2.47 (8) | 0.78 (10) | 1.07 (11) |
| p = 1 × 10–5 | p = 6 × 10–6 | p = 0.03 | |||||
| AT3G16450 | jacalin family | 0.96 | 2.20 (4) | 0.64 | 0.75 (4) | 1.38 (4) | |
| p = 1 × 10–4 | |||||||
| AT3G16460 | jacalin family | 0.65 (5) | 1.36 (8) | 0.91 (6) | 1.35 (7) | 0.80 (11) | 1.45 (16) |
| p = 5 × 10–6 | p = 8 × 10–4 | p = 2 × 10–11 | |||||
| AT3G05900 | neurofilament protein-related | 0.64 (10) | 1.21 (11) | 0.91 (4) | 1.29 (4) | 0.70 (8) | 1.44 (10) |
| AT4G20260 | DREPP family | 0.95 (6) | 1.24 (8) | 0.80 (3) | 1.71 (6) | 0.93 (7) | 1.15 (5) |
| p = 0.009 | p = 0.008 | p = 0.006 | |||||
a
The number of unique peptides whose ratios were averaged to calculate the total protein ratio are indicated in parentheses.
b
Two sample, two-tailed Student t tests were used to determine statistical significance of reciprocal protein ratios if three or more unique peptides were detected in each sample. A third experiment was performed comparing Ntapi:gAHA1b and wildtype plants to distinguish between effects specific to Ntapi:AHA1 expression versus transformation artifacts.