Table 4. Successive Purification of Ntapi:AHA1 from Liquid-Grown Seedlings.
| step | fraction | spectral Ctsa | NSAFb | fold enrichmentc |
|---|---|---|---|---|
| 1 | microsomes | 75 | 0.0012 | |
| 2 | non-PM membranes | 63 | 0.0010 | |
| 3 | plasma membranes | 273 | 0.0051 | 4.25 |
| 4 | IgG bound, TEV-eluted (mild washd) | 99 | 0.0175 | 14.6 |
| 5 | IgG bound, TEV-eluted (stringent washe) | 100 | 0.5639 | 470 |
a
Spectral counts of a representative sample as determined by “number of assigned spectra” in Scaffold.
b
Normalized spectral abundance factor calculated according to the method of Zhang et al.59
c
Enrichment of Ntapi:AHA1 from microsomal samples as determined by increasing NSAF values.
d
Twice with 100× volume 1% NP40, once with 100× volume 1% NP40, 0.01% SDS.
e
Three times with 100× volume 0.1% SDS, 150 mM NaCl.