Table 6. Response of Ntapi:AHA1-Copurifying Proteins to in Vivo Treatments.
accession | annotation | treatment | 14NTAP+/15NTAP–a | 14NTAP–/15NTAP+b |
---|---|---|---|---|
AT2G18960 | AHA1 | FCc | 0.93 (8) | 0.98 (6) |
p = 0.74 | ||||
IAAd | 1.00 (5) | 1.14 (7) | ||
p = 0.52 | ||||
AT5G62670 | AHA11 | FC | 1.10 (3)e | 0.67 (3) |
p = 0.03f | ||||
AT4G09000 | GRF1 | FC | 1.41(4) | 0.95 (4) |
p = 0.006 | ||||
AT1G78300 | GRF2 | FC | 1.13 (3) | 0.80 (3) |
p = 0.003 | ||||
AT5G38480 | GRF3 | FC | 1.25 (4) | 0.76 (5) |
p = 0.003 | ||||
AT3G45780 | PHOT1 | IAA | 0.87 (2) | 1.17 (5) |
AT2G26080 | ATGLDP2 | IAA | 1.23 (3) | 0.94 (3) |
p = 0.02 | ||||
AT3G23990 | HSP60 | IAA | 1.21 (4) | 0.81 (2) |
Liquid-grown Ntapi:gAHA1 seedlings: 14N seedlings treated, 15N seedlings mock treated.
Reciprocal experiment: 14N seedlings mock treated, 15N seedlings treated.
Fusicoccin treatment: 1 μM for 30 min.
Indole-3-acetic acid treatment: 1 μM for 15 min.
Number of unique peptides included in protein ratio average are indicated in parentheses.
Statistical significance is based on two-sample, two-tailed Student’s t test.