Table 1.

S. cerevisiae strains and plasmids used in this study.

Strain	Genotype	Source
PLY062	W303α	(Nasmyth et al., 1990)
PLY521	W303α, ypk1::TRP	This study
PLY1083	W303α, except ypk1::TRP1 ypk2::HIS3 + [pPL216]	(Niles et al., 2012)
PLY1098	W303α, except ypk1::TRP1 ypk2::HIS3 + [pPL220]	This study
PLY1134	W303α, except avo3-30-MYC:TRP1	(Niles et al., 2012)
PLY1360	W303α, except ypk2::HIS3	This study
INA17-4D	MATa ura3 leu2 his2 trp1 ade1	(Inagaki et al., 1999)
INA106-3B	INA17-4D, except pkh1 D398G pkh2::Leu2	(Inagaki et al., 1999)
PLY1501	LHYa, except lac1::HIS lag1::TRP + [pPL547]	This study
PLY1527	W303α, except ypk1::TRP1 ypk2::HIS3 yap1::KanMX + [pPL220]	This study
PLY1528	W303α, except ypk1::TRP1 ypk2::HIS3 rho0 + [pPL220]	This study
PLY1529	W303α, except ypk1::TRP1 ypk2::HIS3 tpk3::KanMx + [pPL220]	This study
PLY1530	W303α, except ypk1::TRP1 ypk2::HIS3 tpk3::KanMx rho0 + [pPL251]	This study
PLY1533	W303α, except ypk1::TRP1 ypk2::HIS3 fpk1::KanMx + [pPL220]	This study
PLY1534	W303α, except ypk1::TRP1 ypk2::HIS3 dnf3::markerless dnf2::NAT dnf1::KanMx + [pPL220]	This study
PLY1535	W303α, except ypk1::TRP1 ypk2::HIS3 fpk1::NAT tpk3::KanMx + [pPL251]	This study
PLY1536	W303α, except ypk1::TRP1 ypk2::HIS3 fpk1::KanMx rho0+ [pPL220]	This study
PLY1537	W303α, except ypk1::TRP1 ypk2::HIS3 yap1::KanMx fpk1::NAT + [pPL220]	This study
PLY1551	W303α, except fpk1::KanMx	This study
PLY1555	W303α, except PIL1-mCherry:KanMx + [pPL580]	This study
PLY1556	W303α, except ypk1::TRP1 ypk2::HIS3 lcb4::KanMx + [pPL220]	This study
PLY1558	W303α, except ypk1::TRP1 ypk2::HIS3 fpk1::KanMx lcb4::markerless + [pPL220]	This study

Plasmid	Vector	Insert/ORF	Source
	pRS315		(Sikorski and Heiter, 1989)
	pRS316		(Sikorski and Heiter, 1989)
	pRS425		(Sikorski and Heiter, 1989)
	pPL187		(Niles et al., 2012)
	pPL420		(Niles et al., 2012)
pPL215	pPL187	YPK1	(Niles et al., 2012)
pPL216	pRS316	YPK1	This study
pPL220	pRS316	YPK1L424G	This study
pPL240	pPL187	YPK1D242A	(Niles et al., 2012)
pPL251	pRS315	YPK1L424G	(Niles et al., 2012)
pPL433	pPL420	YPK1	(Niles et al., 2012)
pPL491	pPL420	YPK1S644A/T662A	This study
pPL530	pPL420	YPK1T504A	This study
pPL547	pRS315	pTet-LAG1	This study
pPL580	p416Met25	SLM1-GFP	This study
pPL582	pPL187	PDE2	This study
pPL585	pRS425	prCUP-6xMYC-cki12–200 (S125/130A)	This study