Oxidative stress induced cell death in oligodendrocytes and oligodendrocyte precursors in prolonged cerebral hypoperfusion mice. A, Numbers of single-stranded DNA (ssDNA)–positive (ssDNA+) cells in corpus callosum. B, ssDNA staining with a oligodendrocyte precursor cell marker (PDGF-R-α) or a mature oligodendrocyte marker (GST-pi) in the vehicle-treated group at day 28. Scale bar, 10 μm. C, Representative images of GST-pi staining at day 28. Scale bar, 25 μm. D, Numbers of GST-pi–positive (GST-pi+) cells in the lateral side of corpus callosum at day 28. Values are mean±SD of n=5. Ed indicates edaravone treatment group; GST, glutathione S-transferase; PDGF-R-α, platelet-derived growth factor-receptor-α; and Ve, vehicle. *P<0.05.