Table 2. Kinetic Parameters for the Binding of Hemin to wt and Mutant HasAp Proteins at 4 °C.
wt | H32A | Y75A | H83A | |
---|---|---|---|---|
Kd (μM) | 35 | 61 | 129 | 30 |
kcoord1 (s–1) | 552 | 830 | 539 | 118 |
kcoord2 (s–1) | 5.2 | n.o. | 14.4 | 20.5 |
wt | H32A | Y75A | H83A | |
---|---|---|---|---|
Kd (μM) | 35 | 61 | 129 | 30 |
kcoord1 (s–1) | 552 | 830 | 539 | 118 |
kcoord2 (s–1) | 5.2 | n.o. | 14.4 | 20.5 |