Table 1. Marineosin Gene Cluster and Proposed Functions.
| gene | length (aa) | proposed function | nearest NCBI BLAST hit (identity, similarity) |
|---|---|---|---|
| marD | 300 | RedD, putative transcriptional regulator | Streptomyces coelicolor A3(2) NP_629999 (56%, 67%) |
| marX | 963 | RedX, polyketide synthase | Streptomyces coelicolor A3(2) NP_630000 (59%, 69%) |
| marW | 387 | RedW, acyl-CoA dehydrogenase | Streptomyces coelicolor A3(2) NP_630001 (78%, 86%) |
| marY | 105 | RedY, proposed in binding MBC and HBP bipyrrole intermediates | Streptomyces coelicolor A3(2) NP_630002 (58%, 70%) |
| marZ | 216 | RedZ, response regulator | Streptomyces coelicolor A3(2) NP_733677 (54%, 71%) |
| marV | 388 | RedV, hypothetical protein | Streptomyces coelicolor A3(2) NP_630003 (53%, 64%) |
| marU | 279 | RedU, putative 4′-phosphopantetheinyl transferase | Streptomyces coelicolor A3(2) NP_630004 (59%, 68%) |
| marT | 295 | Hypothetical protein | Streptomyces coelicolor A3(2) NP_630005 (65%, 76%) |
| marR | 409 | RedR, 3-oxoacyl-ACP-synthase II | Streptomyces coelicolor A3(2) NP_630007 (79%, 88%) |
| marQ | 110 | RedQ, acyl carrier protein | Streptomyces coelicolor A3(2) NP_630008 (67%, 76%) |
| marP | 333 | RedP, 3-oxoacyl-ACP synthase | Streptomyces coelicolor A3(2) NP_630009 (75%, 84%) |
| marO | 87 | RedO, putative peptidyl carrier protein | Streptomyces coelicolor A3(2) NP_630010 (72%, 81%) |
| marN | 632 | RedN, 8-amino-7-oxononanoate synthase | Streptomyces coelicolor A3(2 NP_630011 (72%, 79%) |
| marM | 531 | RedM, peptide synthase | Streptomyces coelicolor A3(2) NP_630012 (69%, 77%) |
| marL | 2253 | RedL, polyketide synthase | Streptomyces coelicolor A3(2) NP_630013 (60%, 69%) |
| marK | 355 | RedK, oxidoreductase | Streptomyces coelicolor A3(2 NP_630014 (72%, 81%) |
| marJ | 277 | RedJ, thioesterase | Streptomyces coelicolor A3(2) NP_630015 (67%, 73%) |
| marI | 346 | RedI, methyltransferase | Streptomyces coelicolor A3(2) NP_630016 (71%, 84%) |
| marH | 900 | RedH, phosphoenolpyruvate-utilizing enzyme | Streptomyces coelicolor A3(2) NP_733678 (70%, 80%) |
| marG | 451 | RedG, oxidase | Streptomyces coelicolor A3(2) NP_630017 (64%, 80%) |
| marE | 317 | Hypothetical protein, Putative acyltransferase | Streptomyces coelicolor A3(2) NP_630019 (78%, 87%) |
| marAa | 155 | Premarineosin reductase | Streptomyces hygroscopicusYP_006247081 (56%, 73%) |
a
Annotation is based on gene replacement experiments detailed in this study.