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(a) Scheme of Bind-n-Seq method.30 Polyamide–biotin conjugate is incubated in a genome-sized library of all possible 21mers, enriched, sequenced, and the resulting data set analyzed with motif-finding software.30 (b) Polyamide 1 could potentially bind in the forward orientation or the reverse orientation. The highest affinity binding sequence of 1 is the reverse orientation binding 5′-GCGC-3′.
