Table 1. Preferred Binding Orientations of Polyamides 1–8 Were Queried with Bind-n-Seq to Generate the Highest Affinity Sequence Motif^a.

^aPolyamide-mediated thermal stabilization (ΔT_m) of 12 base pair oligonucleotides of the forward (5′-CGCG-3′) and reverse (5′-GCGC-3′) sequences were used to validate the revealed motifs. Melting temperatures reflect the mean and standard deviation of quadruplicate measurements.