(A) Partial
sequence alignment of the zinc finger domains of human
JAZ with each other and with those of Xenopus laevis ZFa. The sequences are aligned on the highly conserved zinc-binding
cysteine and histidine residues in each finger, which are colored
yellow and blue, respectively. Sequence identity and similarity between
fingers are indicated by colored blocks: green for hydrophobic, blue-green
for aromatic, light blue for lysine and arginine, red for aspartate
and glutamate, and pink for small and hydrophilic residues. Residue
numbers in ZF1–4 correspond to the JAZ sequence, and for ZFa,
ZF5–7 correspond to the ZFa sequence. Secondary-structure elements
present in the zinc finger domains are indicated at the bottom of
the figure (arrow, β-strand; box, α-helix). (B) Schematic
representation showing the length of the JAZ zinc finger constructs
and the N- and C-terminal residue numbers.