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. 2014 Feb 13;53(9):1495–1510. doi: 10.1021/bi401675h

Figure 2.

Figure 2

(A) EMSA gels showing binding of full-length (ZF1–4) and two-finger (ZF12, ZF23, and ZF34) JAZ constructs to full-length VAI RNA. In each gel, the first lane is free RNA. In the second lane, the protein concentration is 20 nM (4 nM for ZF34). In each subsequent lane, the protein concentration is doubled until [JAZ] = 5 μM (1 μM for ZF34) in the tenth lane. (B) EMSA gels showing binding of JAZ ZF1–4, ZF12, ZF23, and ZF34 to T57A RNA. In each gel, the first lane is free RNA. In the second lane, the protein concentration is 20 nM (4 nM for ZF34 and ZF23). In each subsequent lane, the protein concentration is doubled until [JAZ] = 5 μM (1 μM for ZF34 and ZF23) in the tenth lane. The figures of the RNA structures were produced using Mfold.38 Full sequences with RNA bases shown in larger type are given in Supporting InformationFigures S2 and S3. The JAZ constructs are identified with colors corresponding to those in Figure 1B.