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Verification of the function of Fma-ABM, Fma-C6H, and Fma-MT. (A) LC–MS detection of 1 upon expression of Fma-ABM in S. cerevisiae BJ5464-NpgA with supplement of 4. S. cerevisiae control is untransformed BJ5464-NpgA. (B) LC–MS analysis of in vitro assays of yeast microsomes containing Fma-ABM toward 4. (C) LC–MS analysis of in vitro assays of Fma-C6H with 6; and combined Fma-C6H and Fma-MT with 6, respectively.
