Figure 5.
Products of Fma-P450 when reconstituted in S. cerevisiae. (A) LC–MS metabolic profiles of coexpression of Fma-TC (encoded by Af520), Fma-P450 (encoded by Af510), and AfCPR. Compounds 13, 15, 16, 18, and 19 are present at trace quantities and are not shown. (B) LC–MS analysis of coexpression of Fma-P450 and AfCPR in S. cerevisiae BJ5464-NpgA with supplement of 3; (C) same as trace B but with supplement of 8. (D) Elucidated structures of compounds 8–19.